Authentication of M14 melanoma cell line proves misidentification of MDA-MB-435 breast cancer cell line

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Authentication of M14 melanoma cell line proves misidentification of MDA‐MB‐435 breast cancer cell line

A variety of analytical approaches have indicated that melanoma cell line UCLA-SO-M14 (M14) and breast carcinoma cell line MDA-MB-435 originate from a common donor. This indicates that at some point in the past, one of these cell lines became misidentified, meaning that it ceased to correspond to the reported donor and instead became falsely identified (through cross-contamination or other mean...

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MDA-MB-435 and M14 cell lines: identical but not M14 melanoma?

A controversy has arisen over the past several years about the true origin of the human MDA-MB-435 cell line. Originally described as a human breast cancer cell line, subsequent expression array studies instead suggested a gene expression profile consistent with a melanoma origin. Subsequent karyotype and comparative genomic hybridization studies supported the idea that current stocks of both M...

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Comment Re: MDA-MB-435 and M14 cell lines: identical but not M14 Melanoma?

In support of the recent article by Chambers (1), we wish to share our data on the origin of the MDA-MB-435 and M14 cell lines. Chambers claims that it is most plausible that both cell lines represent MDA-MB-435 based on two X chromosomes in the MDAMB-435 karyotype, whereas M14 had been derived from a man (1). However, karyotyping does not discriminate between paternal and maternal alleles and ...

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ژورنال

عنوان ژورنال: International Journal of Cancer

سال: 2017

ISSN: 0020-7136

DOI: 10.1002/ijc.31067